《植物生理学报》 2017, 53(6): 1104-1108
通信作者:袁德义;E-mail: yuan-deyi@163.com
摘 要:
筛选并利用优化的染色和透明实验方法, 结合激光共聚焦扫描显微镜观察油茶成熟胚囊内部结构。结果表明, 采用1%曙红染色1 h并用水杨酸甲酯透明5 d是观察油茶成熟胚囊较好的实验处理方案, 胚囊内细胞轮廓清晰, 对所需观察的目标有很好的表现力。基于该优化体系, 构建了油茶胚囊的三维结构, 为油茶及山茶属植物胚囊发育、受精机理等方面的研究提供了更加快速、高效的观察方法。关键词:油茶; 胚囊; 三维结构; 激光共聚焦扫描显微镜
收稿:2017-02-13 修定:2017-04-19
资助:贵州省森林培育及生态建设重点学科人才基地建设项目(黔人领发[2009]9号)、贵州省高层次创新型人才培养“十层次”人才项目(黔科合人才[2015]4003号)、提高马尾松用材林林分质量关键技术研究与示范(黔林科合[2010]03)和国家自然科学基金(31170639)。
Corresponding author: YUAN De-Yi; E-mail: yuan-deyi@163.com
Abstract:
The internal structure of mature embryo sac in Camellia oleifera was observed using confocal laser scanning microscope, based on choosing and utilizing the optimized staining and clearing experimental methods. The experiment methods, the samples were stained with 1% eosin solution for 1 h and treated with methyl salicylate transparent material for 5 d for embryo sac observation, was better than others. The embryo sac clearly showde cell configuration. According to the optimized experimental methods, the three-dimentional structure of C. oleifera embryo sac was reconstructed for the first time. The results provided an effective method for researching the development of embryo sac and the fertilization mechanism in C. oleifera.Key words: Camellia oleifera; embryo sac; three-dimentional structure; confocal laser scanning microscope
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